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Related post: truncation of E has implications for the development of effective subunit vaccines for dengue as well as other flavivirus diseases. For these reasons, we constructed a total of 8 recombinant baculoviruses that expressed 80% E or full-length E of 3 different dengue virus serotypes (type 4, 2, and 3) and Japanese B encephalitis (JE) virus. The baculovirus-expressed 80% E proteins of dengue type 4, 2, 3 and JE viruses was secreted into the medium fluid of the infected cells and was stable. Most animals immunized with the intracellular form of 80% E or the control 93% E developed Voltarol Uk signs of encephalitis but none succumbed to the lethal challenge. On the other hand, none of the mice immunized with extracellular forms of 80% E showed encephalitic symptoms following dengue virus challenge suggesting that these animals developed a higher level of resistance. Analysis of sera Buy Voltarol from immunized mice confirmed that extracellular 80% E induced a 3- to 5-fold higher antibody response than that of intracellular 80% E Voltarol 50mg of 93% E. Similar results were obtained in Voltarol Cream the case of dengue type 2 and 3. Immunization of rhesus monkeys with these E proteins expressed by recombinant Buy Voltarol Tablets baculoviruses is in progress. 10-74 PHS 6040 (Rev. 1/84) "^^^ oCMOmBa prhtmg omtt: in o«mh DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBUC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00370-10 LID PERIOD CX)VERED October 1, 1991 to September 30, 1992 TFTLE OF PROJECT (80 characters or less. Tide must fit on one line Voltarol Emugel between the tmrders.) Studies of Simian Immunodeficiency Virus (SIV) Infection of Macaques PRINCIPAL INVESTIGATOR (Ust other prolessional personnel below the Principal Investigator.) (Name, title, laboratory, and Institute ainiiation) PI: Vanessa Hirsch, D.V.M., D.Sci. Visiting Scientist LID, NIAID Others: Simoy Goldstein, Ph.D. Expert LID, NIAID Robert M. Chanock, M.D. Chief LID, NIAID Tiffany Hamm Biologist LID, NIAID COOPERATING UNITS (Ifarw) LIG, NIAID, NIH, Bethesda, MD (Fauci, Koenig); Georgetown University (Fomsgaard, Gerin, Johnson, London); Delta Primate Center (Murphy-Corb); Southwest Foundation (Allen) LAB/BRANCH Laboratory of Infectious Diseases SECTION Immunodeficiency Viruses Section INSTrrUTE AND LOCATION NIAID, NIH, Bethesda, MD 20892 TOTAL MAN- YEARS: 8.0 PROFESSIONAL: 4.0 4.0 CHECK APPROPRIATE BOX(ES) n (a) Human subjects D (b) Human tissues H (c) Neither D (a1) Minors n (a2) Interviews SUMMARY OF WORK (Use standard Voltarol Emulgel 100g unreduced type. Da not exceed the space provided.) Major emphasis was given to: (1) phylogenetic analysis of simian immunodeficiency virus (SrV) strains of African origin, (2) elucidation of viral determinants of SIV pathogenicity, and (3) evaluation Voltarol Tablets 50mg of various SIV vaccine strategies. An SrV isolate from a Sykes monkey was characterized and shown to be the prototype of a new, distinct primate lentivirus group. Genetic determinants of the acutely lethal SIVsm/PBj mutant were identified using chimeric viruses produced by exchange of segments of this mutant and the minimally pathogenic SFVsm Voltarol Gel Uk H4 clone. Virulence of SIVsm/PBj is complex and multigenic, involving regions of the envelope glycoprotein (env) as well as regions of gag and/or pol. One of five full-length molecular clones of SrVsm derived from an infected monkey that developed AIDS proved to be infectious. This clone will be studied in detail during the coming year. SIVsm clones with distinct cell tropism were used to produce infectious chimeras. Analysis of these chimeras indicated that macrophage tropism resides in the V3 loop region of env. Finally, an intensive analysis of virologic Voltarol 75mg and immunologic events during the very earliest stage of SIV infection in vivo was initiated. Our Voltarol Gel initial effort to develop an inactivated SIV vaccine was highly successful. Monkeys vaccinated with a psoralen-inactivated SIV suspension, derived from a proviral molecular clone, were completely resistant to challenge with homologous as weU as heterologous (20% env sequence divergence) cell-free virus. IgG purified from the plasma Voltarol Emulgel P of vaccinated monkeys protected naive monkeys from cell-free homologous SIV. However, the vaccine did not protect against challenge with cell-associated SIV but did appear to slow progression of disease. 10-75 PHS 6040 (Rev. 1/84) Voltarol 50mg Tablets usGCWSWiefrPBiniBOfFKi: wio«tM» DEPARTMENT OF HEALTH AND Voltarol Retard 75mg HUMAN SERVICES - PUBUC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00616-02 LID PERIOD COVERED October 1, 1991 to September 30, 1992 TITLE OF PROJECT (80 Characters or less. VHe must Ht on one line between the borders.) Feline Immunodeficiency Virus Infection as a Model for Human AIDS PRINCIPAL INVESTIGATOR (Ust other Voltarol Emulgel prolessional personnel tjelow the Principal Investigator.) (Name, tiHe, laboratory, and Institute atSliaHon) PI: Raymond Langley, Ph.D. Visiting Associate LID, NIAID Others: Vanessa Hirsch, D.V.M., D.Sci. Visiting Scientist LID, NIAID Diane Adger-Johnson Biologist LED, NIAID Robert Goeken Biologist LID, NIAID COOPERATING Voltarol Tablets UNITS (II any) Georgetown University (Johnson, Hirsch, Goff, Albert); NCI (O'Brien); Florida Fresh Water Fish and Game Commission (Roelke); Center for Retroviral Research, Ohio State University (Lafrado) LASmRANCH Laboratory of Infectious Diseases SECTION Immunodeficiency Viruses Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, MD 20892 TOTAL MAN- YEARS: 2.0 PROFESSIONAL:
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